Journal: bioRxiv
Article Title: Optimizing C14120-based LNPs for in vitro and in vivo mRNA delivery
doi: 10.1101/2025.09.02.673705
Figure Lengend Snippet: Cell-type and origin-dependency of in vitro mCherry expression for individual LNPs measured 24 hours post-transfection. (a) Histograms showing mCherry expression in bEnd3 (blue) and AML12 (red) cells upon transfection with LNPs formulated with different types of PEG-lipids (TPGS and DSPE-PEG) in comparison to respective untransfected sample (Ctrl). (b-c) mCherry expression levels, respective of data shown in a), but including a Lipofectamine 2000 (LIPO) transfection control. (c) mCherry expression level as a function of PEG-lipid percentages (DSPE-PEG and TPGS) in ARPE-19, Hep G2, AML12 and HEK293-H. Data are presented as Mean+-SD (n = 3); * p < 0.05 , ** p < 0.01 , *** p < 0.001 . Significance was determined by independent t -test.
Article Snippet: 2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE– PEG-2000), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) were purchased from Avanti Polar Lipids.
Techniques: In Vitro, Expressing, Transfection, Comparison, Control